Majority of the corrosion inhibition studies of microbially influenced corrosion (MIC) have been carried out in the static mode conditions. Considering the constant flow similar to industrial pipelines, the dynamic systems for the corrosion inhibition analysis provide more accurate investigation of pipeline corrosion. Towards this aspect, we have analyzed the inhibition of MIC induced by chitosan@lignosulfonate (CS@LS) nanospheres in dynamic water injection systems using custom-made flow cell with a flow rate of 1 L/min. The designing and fabrication of flow cell have been made in a way that carbon steel coupons are placed in the bottom of flow cell. From the scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS) analysis, the sulfate reducing bacteria (SRB) induced corrosion is visible in SS400 carbon steel coupons and the corrosion products are FeS, Fe2O3 and organic sulfur as a result of SRB activity, however, the intensity of these peaks reduced in the presence of CS@LS. The electrochemical impedance analysis showed that the CS@LS exhibited a maximum of 84% corrosion inhibition. This work provides an insight into the use of CS@LS nanosphere in realistic practical applications such as oil and gas plants to prevent the damages caused by MIC.