A Nb4C3Tx (MXene)-modified glassy carbon electrode was used for the electrochemical detection of Pb2+ ions in aqueous media. The sensing platform was evaluated by anodic stripping analysis after optimizing the influencing factors such as pH, deposition potential and time. The large interlayer spacing, high c lattice parameter and higher conductivity of Nb4C3Tx compared to other MXenes enhance the electrochemical detection of Pb2+. The developed sensor can reach a detection limit of 12 nM at a potential ∼-0.6 V. Additionally, the developed sensor showed promising selectivity in the presence of Cu2+ and Cd2+ and stability for at least 5 cycles of continuous measurements with good repeatability. This work demonstrates the potential applications of Nb4C3Tx towards the development of effective electrochemical sensors.