Computational Fluid Dynamics simulations are performed to study mixture preparation in the intake manifold of a small engine with Port Fuel Injection. Data from laser-based experiments on two injectors are used as inputs to the spray sub-model. The results reveal improved in-cylinder fuel distribution when the spray is directed onto the intake valve. The Sauter Mean Diameter of the spray is observed to have an insignificant effect during open valve injection, while the targeting is found to have a large influence. The methodology developed as part of this work can be used to further optimize injection parameters in such engines. Copyright © 2012 Inderscience Enterprises Ltd.