We describe the design and characterization of a DNA polymerase-nanopore protein construct on an integrated chip. This system is able to incorporate all four DNA bases and distinguish single tagged nucleotide addition in real-time. Coupling protein catalysis and nanopore-based detection to an electrode array could provide the foundation of a highly-scalable, single-molecule, electronic DNA sequencing platform.

Mintu Porel

Department of Chemistry

P.B. Stranges

M. Palla

S. Kalachikov

J. Nivala

M. Dorwart

A. Trans

S. Kumar

M. Chien

C. Tao

Indian Institute of Technology Palakkad&nbsp;(IIT Palakkad or IIT PKD) is a public autonomous&nbsp;engineering&nbsp;and&nbsp;research&nbsp;institute located in&nbsp;Palakkad,&nbsp;Kerala. It was&nbsp;one of the five new&nbsp;IITs&nbsp;proposed in the&nbsp;2014 Union budget of India. The campus was inaugurated on 3 August 2015. The institute has 94 faculty, 978 students, and 63 non-teaching staffs.

IT Palakkad currently offers four-year Bachelor of Technology (B.Tech) and two years MS programmes in 8 different engineering disciplines including&nbsp;Computer Science, Electrical, Mechanical, Civil among others.

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IIT Palakkad

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Design and characterization of a nanopore-coupled polymerase for single molecule DNA sequencing by synthesis on an integrated electronic array

Journal	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Publisher	Chemical and Biological Microsystems Society